Reporter
Part:BBa_K199030:Experience
Designed by: Olivia Ho-Shing Group: iGEM09_MoWestern_Davidson (2009-07-11)
In order to have a visible expression of RFP or GFP, we constructed parts with RBS-RFP under the control of 4 different commonly-used promoters:
- pBad (Potential induction with L-arabinose)
- pLac (Potential induction with IPTG)
- pLacIQ (Potential induction with IPTG)
- pTet
We found that pLac induced with IPTG caused the greatest expression of RFP. This construct, pLac-RBS-RFP, was chosen as our control construct, representing 100% suppression of the engineered frameshift.
- Notice the faint pink color with the pBad promoter only if Arabinose was added (tube number 3). We wanted to produce a part that gave a weak signal compared to all the strong promoter versions that existed.
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